LAB 11 OBJECTIVES
1) Identification of unknown bacteria
(Period 4).
a)
Obtain
all the cultures you prepared from period 3.
Use these cultures to perform the following tests. Record all results on your Descriptive Chart.
i)
Sugar
fermentation tests (phenol red glucose broth, phenol red lactose broth, phenol
red sucrose broth, & phenol red mannitol broth)
ii)
Nitrate
reduction test (nitrate broth)
iii)
Urea
hydrolysis test (urea broth)
iv)
Phenylalanine
deaminase test (phenylalanine agar)
v)
Hydrogen
sulfide test (Kligler iron agar)
vi)
Oxidase
test (tryptic soy agar)
vii) Catalase test (tryptic soy agar)
viii) Starch hydrolysis test (starch agar)
ix)
Casein
hydrolysis test (skim milk agar)
x)
Lipase
test (spirit blue agar)
2) Identification of unknown bacteria
(Period 5).
a) Using all the data recorded on your
Descriptive Chart for your unknown, determine the identity of this bacterium by
using the following resources:
-
Lab
manual (chapter 41 separation outlines)
-
Bergey’s
Manual of Determinative Bacteriology
-
Probabilistic
Identification of Bacteria Computer Program
b) Submit your final analysis to your
instructor.