LAB 8 OBJECTIVES
1) Cultivating microbes (part 2). Know the steps involved in preparing sterile
nutrient broth tubes and nutrient agar deep tubes, slant tubes, and
plates. Be familiar with the general
maintenance, incubation, and preservation of stock cultures and working
cultures.
2) Be able to calculate population
counts from nutrient broth cultures of bacteria (E. coli) and from a food sample.
Be familiar with all the procedures and materials used to perform
quantitative plating (standard plate count).
Methods include:
a. knowing how to properly use and
handle dilution pipettes
b. knowing how to prepare blanks of
known dilutions
c. knowing how to properly prepare
plates using melted nutrient agar pours
d. knowing how to use a
e. knowing how to mathematically calculate the
number of colony forming units (CFUs) per milliliter
from a bacterial culture or number of CFU’s per gram
from a food sample.