LAB 7 OBJECTIVES

1) Cultivating microbes (part 1). Know the different nutritional and physical requirements that must be considered when culturing microbes in the laboratory. Become familiar with the different types of culture media used to help meet these requirements. Understand the useful properties of agar and other solidifying agents. Know the general uses and differences between synthetic, complex, enriched, selective and differential media.

2) From prepared slides and specimens, be able to locate and identify specific parasitic worms in their egg, larval, or adult stage. For each worm species:

a. be able to classify them into their proper phylum and class

b. know the name of the disease they cause

c. know how each parasite is transmitted to humans

3) Macroscopically observe various species of yeasts and molds cultures prepared on Petri plates containing Sabouraud’s dextrose agar. Know why this medium is used for culturing fungi. Become familiar with each species cultural characteristics (i.e., colors, textures, shapes, and size of colonies).

4) Prepare wet mount slides of different species of yeast and observe them microscopically. Become familiar with the general microscopic features of yeasts such as budding cells, pseudohyphae, and sporangia. Along with their cultural characteristics, use these microscopic features to identify each species.

5) Be able to prepare microscope slide cultures of various mold species. Know what materials are required and how they are used to perform this technique. After incubation, microscopically observe these slide cultures. Become familiar with the following morphological features: sporangiophore, sporangium, sporangiospores, zygospore, non-septate hyphae, conidiophore, conidia, and septate hyphae. Be able to use these microscopic features, in addition to their cultural characteristics, to identify each species of mold.